For diagnosis of swine influenza A infection, respiratory specimen would generally need to be collected within the first 4 to 5 days of illness (when an infected person is most likely to be shedding virus). However, some persons, especially children, may shed virus for 10 days or longer.
Sample Collection & Laboratory Diagnosis
· Preferred respiratory samples Nasal, Nasopharyngeal & throat swab
· Collection- on vaccine transport media
· Availability- with area & regional coordinator
· Storage of Samples : should be at 2-8⁰C until can be placed at -70⁰C.
· Transportation of Samples : dry ice in triple packaging. All samples should be labeled clearly and include patient’s complete information
· Laboratory biosafety measures should be followed for collections, storage, packaging and courier/ shipping of influenza samples.
· Available Laboratory tests:
- Rapid Antigen Tests: not as sensitive as other available tests.
- RT-PCR, Virus isolation, Virus Genome Sequencing, Four-fold rise in swine influenza A (H1N1) virus specific neutralizing antibodies